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KMID : 0613820210310080761
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Journal of Life Science
2021 Volume.31 No. 8 p.761 ~ p.770
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Optimization of Medium to Improve Protease Production Using Response Surface Methodology by Bacillus amyloliquefaciens SRCM115785
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Yang Hee-Gun
Ha Gwang-Su
Ryu Myeong-Seon
Park Se-Won
Jeong Ho-Jin
Yang Hee-Jong
Jeong Do-Youn
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Abstract
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In this study, the optimal medium composition for enhancing protease production was established by the Bacillus strain isolated from Makgeolli, a traditional fermented food, using the response surface methodology. B. amyloliquefaciens SRCM115785 was selected as the protease producer by productivity analysis and identified by 16S rRNA gene sequencing. Plackett-Burman design (PBD) was introduced to analyze the effect of each component on protease production among the 11 selected medium components. As a result, glucose, yeast extract, and beef extract were finally selected as factors for enhancing protease production. Central composite design (CCD) analysis was designed as a method to determine the optimal concentration of each component for protease production and the concentration of each medium composition for maximum protease production was predicted to glucose 6.75 g/l, yeast extract 12.42 g/l and beef extract 17.48 g/l. The suitability of the experimental model was proved using ANOVA analysis and as a result of quantitative analysis to prove this, the amount of increase was 230.47% compared to the LB medium used as a control. Through this study, the optimization of medium composition for enhancing protease production was established, and based on this, it is expected that it can be efficient use of protease as an industrial enzyme.
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KEYWORD
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Bacillus amyloliquefaciens, optimization, protease activity, response surface methodology
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